Endocrine Abstracts

Introduction: Formation of immunological tolerance to self antigens is an important mechanism that prevents the development of autoimmune diseases.Methods: With the help of molecular genetic and immunofluorescence techniques we investigated the effects of experimental gestational diabetes (EGD) on the level of mRNA expression of autoimmune regulator Aire and differentiation features of Foxp3+ cells in mesenteric lymph nodes in the offspring of...

Introduction: Type 1 diabetes mellitus is a T-cell mediated autoimmune disease characterized by the destruction of β-cells of the pancreas. Numerous studies have demonstrated the key role of FoxP3+ regulatory T-cells in the development of type 1 diabetes.Aim: The aim of research: 1) to determine the expression patterns of transcription factor FoxP3 in the pancreatic lymph node cells in animal model of diabetes mellitus and 2) to assay an effect of m...

Introduction: Changing of expression of pattern-recognition receptors (PRR) of innate immune response by the gut-associated lymphoid tissue (GALT) can play a critical role in an induction and progression of T1DM.Aim: To study the peculiarities of NOD2 receptors in gut-associated lymphoid tissues (GALT) of rats with experimental STZ-induced diabetes mellitus and pentoxifylline (PTX) administration.Methods: Researches were made on Wi...

Introduction: Mesenteric lymph nodes (MLN) is a major transition point for recirculating lymphocytes of GALT. Homing of lymphocytes in MLN is regulated with adressin MAdCAM-1, chemokine receptors CXCR4 and CCR7. Sphingosine-1-phosphate receptors S1PR1 activate T-cell exit from MLN.Methods: We use RT-PCR method for investigating of mRNA expression levels of genes MAdCAM-1, CXCR4, CCR7 and S1PR in MLN of the offspring of rats with experimental gestational ...

Introduction: mTOR is not only a central regulator of lipid metabolism, controlling the processes of adipogenesis and lipolysis, but also a regulator of immunometabolism of immune cells infiltrating the adipose tissue. In its turn, the level of progression of diabetes is largely limited by Treg subpopulation, the complexity and heterogeneity of which is confirmed by the detection of numerous tissue-specific Tregs, including the so-called VAT Tregs (visceral adipose tissue CD4+...